INTRAVITAL IMAGING OF GREEN FLUORESCENT PROTEIN USING 2-PHOTON
LASER-SCANNING MICROSCOPY
by Potter-SM Wang-CM Garrity-PA Fraser-SE (*R)
Caltech,Beckman Inst 13974,Div Biol/Pasadena//CA/91125
GENE
v173 (1) : pp25-31 (1996 Jul 1)
LOC: BIOL
Abstract:
Imaging a fluorophore in a living tissue presents several unique
problems, The fluorescence from the labeled cell(s) may be weak, the
labeled cells may be buried deep within tissue and the presence of a
fluorophore may render the cells photo-sensitive. Two-photon laser-scanning
microscopy (TPLSM) offers several advantages in meeting these challenges.
We show that TPLSM provides greater sensitivity, better resolution and less
photo-bleaching, as compared to confocal laser-scanning microscopy, The
dramatically reduced photo-bleaching makes it possible to image cells
continuously for long periods of time. Therefore, TPLSM allows a safer and
higher-resolution means of imaging living cells labeled with a variety of
fluorophores, including green fluorescent protein.