We seek to answer how animal behavior is set up by the collective behaviors of individual cells, over the entire course of brain development. We want to understand how gene activity can instruct each developing brain cell to move around, change shape, and connect to other cells.
To do this, we study the simple larval nervous system of our closest invertebrate relatives, the tunicates. Tunicates, like us, belong to the Chordate phylum, but have very simple embryos and compact genomes. The laboratory model tunicate Ciona has only 231 neurons (177 in the central nervous system) and is the only chordate with a fully mapped "connectome". We take advantage of this simplicity to understand molecular mechanisms that may underlie human neurodevelopment.
More specifically, we use RNAseq transcriptome profiling to assay global transcriptional dynamics in neural progenitors during Ciona development, and use CRISPR/Cas9 to knock out important transcription factors and their downstream targets to understand how these gene networks control neuronal specification, morphology, physiology, neurotransmitter identity, and connectivity.
Keywords: Neurodevelopment, neuroscience, developmental biology, cell biology, gene regulation, genome engineering, tunicates